亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>HTB-125 Hs 578Bst 人正常乳腺細(xì)胞

HTB-125 Hs 578Bst 人正常乳腺細(xì)胞

簡(jiǎn)要描述:HTB-125 Hs 578Bst 人正常乳腺細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1819

產(chǎn)品分類

Product Category

詳細(xì)介紹

HTB-125 Hs 578Bst 人正常乳腺細(xì)胞

OrganismHomo sapiens, human
Tissuemammary gland/breast
Cell TypeEpithelial, Myoepithelial
Product Formatfrozen
Morphologyfibroblast
Culture Propertiesadherent
Biosafety Level1
Diseasenormal
Age74 years
Genderfemale
EthnicityCaucasian            

HTB-125 Hs 578Bst 人正常乳腺細(xì)胞

Storage Conditionsliquid nitrogen vapor phase
Karyotypemodal number . Both X = 46; range = 42 to 48

This is a diploid human cell line with 46,XX karyotype. Polyploidy occurred at 6.9%chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal.



Derivation

Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126).

Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo.


Receptor ExpressionEpidermal growth factor (EGF)


Comments

No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected.

A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximay 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze.

Complete Growth MediumThe base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Medium Renewal: 2 to 3 times per week



Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

CryopreservationComplete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.


Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日本韩国欧美在线一区| 视频二区国产欧美日韩| 国产美女被狂操到高潮| 国产精品久久久久精品三级中文国| 两性色午夜视频免费网站| 国产在线观看91精品一区| 国产av麻豆全部免费| av在线男人社区日韩| av网址在线免费观看| 一区二区三区乱码av| 亚洲综合香蕉在线视频| 午夜丁香婷婷爽少妇av| av一区二区三区三| 熟女乱熟乱熟妇综合网二区| 夜夜高潮夜夜爽免费观看| 成年自拍视频在线观看| 丰满人妻被猛烈进入中文版| 久久久精品国产亚洲av色哟哟| 日本高清中文精品在线不卡| 天天操天天射夜夜撸| 18禁成年大片免费网站| 激情欧美一区二区三区精品| 丰满人妻少妇精品一区二区三区| 久久国产精品成人免费蜜臀| 一区不卡在线视频免费国产| 亚洲精品不卡一二三区| 亚洲av乱码一区二区三四五六七| 亚洲综合中文字幕经典av在线| 国产黄的网站在线观看| 高级会所口爆视频在线播放视频| 人妻少妇av免费久久蜜臀| 婷婷亚洲五月伊人91| 人妻熟女在线一区二区| 性生活视频在线观看免费| 国产男女做爰在线视频| 在线中文字幕日韩精品| 一级亚洲国产日韩欧美| 91一区二区亚洲嫩草| 老熟妇奂伦一区二区三区| 99久久中文字幕伊人| 国产精品视频一区二区三区网站|