亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>CRL-11610RWPE-2 人前列腺正常細胞

RWPE-2 人前列腺正常細胞

簡要描述:CRL-11610 RWPE-2 人前列腺正常細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-11610
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2834

產(chǎn)品分類

Product Category

詳細介紹

CRL-11610 RWPE-2 人前列腺正常細胞

ATCC® Number:CRL-11610™    Price:
Designations:RWPE-2

Depositors:Michigan State University, National Cancer Institute

Biosafety Level:2 [Cells contain Human Papilloma viral (HPV) sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:CRL-11610 RWPE-2 人前列腺正常細胞epithelial


Source:Organ: prostate
Disease: normal
Cell Type: epithelial


Cellular Products:cytokeratin 18 [46793]

cytokeratin 8 [46793]



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Receptors:androgen receptor, expressed ( [46793] upregulated upon exposure to androgen)

Tumorigenic:Yes

Antigen Expression:kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) ( [46793] upon exposure to androgen)

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8,9.3

TPOX: 8,11

vWA: 14,18



Cytogenetic Analysis:At passage 14, a majority of the cells were in the near diploid range (48-54), with the main population being 51, XY. [46795]

Isoenzymes:AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 0

PGM1, 2

PGM3, 1



Gender:CRL-11610 male

Ethnicity:Caucasian, White

Comments:Tumor Suppressor Gene(s):pRB + [PubMed: 9214605]

RWPE-2 cells were derived from RWPE-1 cells (ATCC CRL-11609) by transformation with Ki-ras using the Kirsten murine sarcoma virus (Ki-MuSV) [PubMed: 9214605]. Epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome to establish the RWPE-1 cell line (ATCC CRL-11609). RWPE-1 cells neither grow in agar nor form tumors when injected into nude mice with or without Matrigel. However, RWPE-2 cells form small colonies in soft agar and form tumors when injected into nude mice [PubMed: 9214605]. Further, a family of tumorigenic cell lines, that mimics multiple steps in prostate cancer progression, was also derived from RWPE-1 cells by exposure to N-methyl-N-nitrsourea (MNU). See the WPE1-NA22 (ATCCCRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell ines.

The depositor reports that the RWPE-1 cell line (ATCC CRL-11609) was screened, and found negative for, Hepatitis B virus, Hepatitis C virus and Human immunodeficiency virus.



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C



Subculturing:CRL-11610 Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).

  3. Add 2.0 to 3.0 ml (to a T-25 flask) or 3.0 to 4.0 ml (to a T-75 flask) of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.

  4. Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS), as appropriate, and aspirate cells by gently pipetting.

  5. Transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 7 minutes.

  6. Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 4 X 10(4) and 8 X 10(4) cells/sq. cm.

Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 days


Preservation:Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:parental cell line:ATCC CRL-11609

purified DNA:ATCC CRL-11610D

derived from same individual:ATCC CRL-2849

derived from same individual:ATCC CRL-2850

derived from same individual:ATCC CRL-2852

derived from same individual:ATCC CRL-2851

derivative:ATCC CRL-2853

derived from same individual:ATCC CRL-2854



References:46792: Webber MM, Rhim JS. Immortalized and malignant human prostatic cell lines. US Patent 5,824,488 dated Oct 20 1998
46793: Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
53180: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636
53181: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337
53182: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152
53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759
53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497
CRL-11610


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲精品免费福利视频| 国产日韩欧美 一区二区三区| 国产精品传媒在线观看网站| 肥臀大屁股av在线播放| 国产福利在线观看午夜| 欧美午夜国产在线观看| 国产一区欧美二区日韩三区| 欧美性生活真实的视频| 人妻av天堂综合一区| 亚洲午夜福利理论片在线| 国产乱一伦一性一情一色| 亚洲午夜精品一区二区久久| 男人午夜激情免费网站| 久久精品久久黄色片看看| 一区二区日韩视频九一蜜桃| 蜜桃少妇人妻一区二区视频| 在线观看一区二区三区国产视频| 精品久久少妇激情视频| 亚洲久久精品中文字幕| 久久久这里只有精品99| 欧美日韩在线国产一区| 亚洲伦理第一页中文字幕| 欧美一级特黄免费大片| 国产高清av免费在线播放| 丝袜美腿亚洲欧美日韩| 国产一区二区三区av在线播放| 精品人妻在线中文字幕| 麻豆深夜激情在线观看| 免费国产中文字幕黄网站| 欧美精品中出一区二区三区| 亚洲码av一区二区三区| 欧美日韩精品免费在线观看| 国产不卡高清视频在线| 欧美激情三级一区二区| 国产精品三级一区二区三区| 亚洲欧美激情啪啪啪| 久久综合婷婷亚洲五月| 白白色发布青青在线视频观看| 91久久国产综合精品女同| 日韩成人在线高清视频| 欧美一区二区三区中文字幕|