亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

簡(jiǎn)要描述:CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問  量:2963

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

ATCC® Number:  CL-188™

Designations:  LS 174T

Depositors:   Northwestern University

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: colon

Tumor Stage: Dukes' type B

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) (The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days.)

interleukin 10 (IL-10) [22511]

interleukin 6 (IL-6)

mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -

Antigen Expression: serologically defined colon cancer antigen 3; Homo sapiens, expressed

HLA A2, B13, B50; Blood type O

Cytogenetic Analysis: 45,X; one X chromosome missing; no other chromosomal aberrations

Isoenzymes:  ADA, 1

ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age:  58 years

Gender:  female

Ethnicity:  Caucasian

Comments: LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subc*ted than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA).

Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles [Pubmed ID: 1262041].

They are negative for p53 antigen expression, but positive for mRNA expression.

LS 174T cells stain positively for cytokeratins.

The line is positive for expression of c-myc, N-myc, H-ras, N-ras.

Myb, and fos oncogenes.

K-ras and sis oncogene expression were not detected.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CL-187

References: 2148: Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041

3524: Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980

22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

32377: Shibata D, et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390

32794: Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
91综合午夜精品福利| 日韩欧美一区二区三区在线| 日本韩国欧美一区在线| 亚洲品质一区二区三区| 欧美亚洲清纯唯美另类| 国产高清视频不卡在线| 国产精品自产在线观看一| 日本大片在线一区二区三区| 亚洲少妇精品视频在线| 亚洲一区日韩精品电影| 一区二区三区毛片免费| 亚洲91精品一区二区三区| 久久精品国产亚洲av品| 亚洲精品中文字幕乱码| 把熟睡的少妇弄到高潮| 日本中文有码在线观看| 男女性视频在线免费观看| 动漫美女视频在线看黄| 亚洲人妖视频在线观看| 日本高清中文精品在线不卡| 人体艺术日韩色噜噜| 国产精品日韩欧美一区二区| 日韩欧美国产精品专区| 国产精品久久久久精品爆| 人妻中文字幕av资源| 午夜福利视频在线观看| 国产白丝诱惑在线视频| 国产精品久久久久大屁股精品性色| 色婷婷一区二区三区四| 国产精品久久99一区| 欧美日韩国产另类久久| 国产精品伊人久久精品| 亚洲成人黄色片在线观看| 丰满少妇亚洲中文字幕| 欧美十日本亚洲激情视频| 国产日韩精品一区二区三区在线| 亚洲成人av毛片在线观看| 热精品韩国毛久久久久久| 东京热男人的天堂视频| 天天操天天射夜夜撸| 日日做日夜夜操天天搞|