C2C12細胞，小鼠成肌細胞

簡要描述：C2C12細胞，小鼠成肌細胞
原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和*培養(yǎng)條件！

產(chǎn)品型號：CRL-1772
廠商性質(zhì)：生產(chǎn)廠家
更新時間：2024-11-21
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細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養(yǎng)基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質(zhì)瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結(jié)直腸腺癌人經(jīng)典小細胞肺癌細胞人小細胞肺癌細胞人結(jié)直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉(zhuǎn)錄病毒包裝穩(wěn)定細胞株小鼠神經(jīng)細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉(zhuǎn)錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結(jié)腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導(dǎo)因子人乳腺癌細胞結(jié)腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導(dǎo)表達穩(wěn)定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產(chǎn)品

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C2C12細胞，小鼠成肌細胞

ATCC^® Number:	CRL-1772™	Price:	$256.00
Designations:C2C12Biosafety Level:1Shipped:frozenMedium Properties:& Serum:oSee PropagationGrowthadherentOrganism:Mus musculus (muse)Morphology:myoblastSource:Strain: C3H Tissue: muscle Cell Type: myoblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. C2C12細胞，小鼠成肌細胞Applications:transfection host (Nucleofection technology from LonzaRoche FuGENE® Transfection Reagents)Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903] The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953] Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] Tested and found negative for ectromelia virus (mousepox).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.Incubate cultures at 37°C.

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C2C12細胞， 小鼠成肌細胞

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C2C12細胞，小鼠成肌細胞