亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

簡(jiǎn)要描述:WM-266-4細(xì)胞, 人黑素瘤細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1676
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1594

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

ATCC® Number:CRL-1676™    Price:$318.00
Designations:WM-266-4Depositors:M HerlynBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth aProperties:adherentOrganism:Homo sapiens (humn)Morphology:epithelial Source:Organ: skin Disease: melanomaCellular Products:proteoglycan antigen characteristic of melanomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.WM-266-4細(xì)胞, 人黑素瘤細(xì)胞Tumorigenic:YesDNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 13 D7S820: 8 THO1: 7,9 TPOX: 8,11 vWA: 15,17Cytogenetic Analysis:The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.Age:58 yearsGender:femaleComments:The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma. Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:                                          Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020derived from same individual:ATCC CRL-1675

















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
av高清不卡一区二区免费在线 | 午夜精品三级一区二区三区| 欧美亚洲另类日韩综合网| 中文字幕日韩av综合在线| 国产精品成人大片在线播放| 日韩三级视频一区二区| 久久亚洲中文字幕精品熟女一区 | 中文字幕一区二区久久综合| 亚洲成人黄色在线网站| 欧美一级免费黄片在线播放| 91欧美精品在线视频| 中文字幕一区二区三天| 农村人妻一区二区三区视频| 国产欧美日本一区二区| 亚洲精品一品区二品区三| 亚洲一区欧美二区日韩三区| 成人在线视频国产自拍| 日韩高清不卡免费视频| 日本中文字幕免费一区| 欧美精品熟妇乱黑人最大| 国产精品女同久久久久久| 成人国产亚洲日本欧美| 怡红院怡春院视频免费看| 97超碰97资源在线| 白白色最新福利在线视频观看| av在线免费播放观看| 内射嫩国产欧美国产日韩欧美| 亚洲av日韩av一区| 91久久福利国产成人精品| 午夜精品久久久免费视频| 综合国产精品久久久久久| 国产青青草成人在线视频| 熟女人妻精品一二三四| 欧美精品一区二区网站| 久久成人a毛片免费观看网站| 亚洲国产天堂久久综合| 亚洲国产视频中文字幕| 国产精品神马午夜福利| 国产高清不卡一区二区| 日韩中字在线一区二区| 背德人妻中文字幕无修|