亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>COLO-320人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡要描述:COLO-320 人結(jié)腸腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:COLO-320
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1929

產(chǎn)品分類

Product Category

詳細(xì)介紹

COLO-320 人結(jié)腸腺癌細(xì)胞

ATCC® Number: CCL-220™

Designations: COLO 320DM

Depositors: GE Moore

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens (human)

Morphology: rounded and refractile

COLO-320 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Cytogenetic Analysis: modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

Add 2.0 to 3.0 of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products

COLO-320



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产白丝扒开做爽爽爽网站| 人妻人人澡人人添人人爽桃色 | 亚洲男人天堂超碰在线| 日韩精品在线观看不卡| 黄色av福利在线网站| 国产一区二区三区不卡av| 中文字幕人妻熟人妻熟丝| 国内熟妇人妻色在线三级| 老色鬼久久亚洲av综合| 欧美日韩福利一区二区三区| 色呦呦视频在线免费观看| 亚洲成av人片青草影院| 九九热在线视频观看最新| 精品一区二区三区推荐| 国产精品国产三级国产不产一地| 欧美精品福利一区二区三区| 日日插天天干夜夜操| 欧美日韩国产在线91| 日韩中文字幕在线首页| 中文字幕成人在线电影| 久久人人97超碰人人爱一久久精品| 亚洲精品一区二区免费看| 老熟妇仑乱换频一区二区| 一级片欧美女人性生活片| 日韩少妇黄色在线观看| 先锋影音女同中文字幕| 99久久精彩免费视频| 亚洲黄色av电影在线| 午夜视频在线观看黄片| 国产亚洲国产av网站在线| 福利视频免费观看欧美| 国产三级在线观看视频| 国产成人自拍激情视频| av影片在线观看不卡| 熟女亚洲一区精品久久| 欧美日韩在线视频一区| 国产精品线路一线路三| 99麻豆久久久精品国产| 成年人正常性生活频率| 欧美日韩免费r在线视频| 成人性生交大片免费看中文|