亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2557Panc 05.04 人胰腺癌細(xì)胞

Panc 05.04 人胰腺癌細(xì)胞

簡要描述:CRL-2557 Panc 05.04 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2557
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1755

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

ATCC® Number: CRL-2557™ Price: $417.00

Designations: Panc 05.04

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

Isolation: Isolation date: May 5, 1995

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type B; Rh+

Age: 77 years adult

Gender: female

Ethnicity: White

Comments: Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended

Medium Renewal: Add media once per week. Fluid change one to two times per week.

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 46 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

CRL-2557


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
色婷婷激情一区二区三区| 日本中文字幕黄色人妻| 91薄丝激情在线播放| 天堂免费av在线播放| 成人午夜福利视频免费观看| 国产亚洲精品久久久9| 操小姑娘国产精品视频| 在线观看国产激情免费视频| 麻豆av永久地址久久精品| 日韩av一区二区国产| 久久热在线观看免费高清 | 黄色国产欧美国产亚洲| 国产av一级二级三级最新精品| 国产精品中文一区二区三区| 国产精品高清另类一区二区三区| 国产传媒在线免费播放| 国内午夜福利精品视频| 亚洲精品视频久久偷拍| 欧美黄片视频在线免费看| 在线观看91高清视频| 精品伊人久久大香线蕉| 熟女乱熟乱熟妇综合网二区| 欧美一区二区三区日韩色| 国产无套内射三级视频| 久久夜色一区二区三区| 日韩中文字幕一二一二区| 欧美日韩精品成人大片| 中文字幕国产精品欧美| 精品国产一区二区三区四不卡在线 | 国产高清av免费观看| 四虎永久精品国产毛片| 免费在线一区二区av| 亚洲中文字幕乱码丝袜在线精品| 欧美日韩另类综合久久久| 91九色蝌蚪国产欧美亚洲| 日韩精品在线观看一二三区| 中文字幕你懂的在线观看| 久久热这里只有精品网站| 一本久久精品午夜福利| 欧美特黄大片在线观看| 亚洲男人天堂最新地址|