亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>HB-82BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

簡要描述:HB-82 BB7.2小鼠 雜交瘤 B淋巴細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞株|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:HB-82
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2082

產(chǎn)品分類

Product Category

詳細(xì)介紹

HB-82 BB7.2小鼠 雜交瘤 B淋巴細(xì)胞 的詳細(xì)介紹

HB-82 BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

ATCC® Number:HB-82™  Price:$399.00
Designations:BB7.2

Depositors:P Parham

Isotype:IgG2b

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))

Morphology:lymphoblast


Source:Cell Type: hybridoma: B lymphocyte;

Cellular Products:immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HB-82

Comments:Animals were immunized with papain solubilized HLA A2 antigen.

Spleen cells were fused with NS-1 myeloma cells.

Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935]

BB7.2 and PA2.1 (ATCC HB-117) recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936]

The antibody cross-reacts with cells from various primate species.

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.



References:950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415

32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756

39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666


ATCC® Number: HB-82™ Price: $399.00

Designations: BB7.2

Depositors: P Parham

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))

Morphology: lymphoblast



Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Comments: Animals were immunized with papain solubilized HLA A2 antigen.

Spleen cells were fused with NS-1 myeloma cells.

Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935]

BB7.2 and PA2.1 (ATCC HB-117) recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936]

The antibody cross-reacts with cells from various primate species.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415

32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756

39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
成年人免费在线观看国产| 激情五月婷婷我有我色| 麻豆成人三级电影在线| 成人色视频免费在线观看| 一区二区三区视频观看在线| 亚洲天堂毛片在线观看| 国内一级片内射免费视频观看| 超碰免费在线公开97| 亚洲一区二区精品天堂| 97在线观看视频免费| 操老熟女一区二区三区| 91中文字幕在线一区| 97青青草免费在线视频| 日本中文字幕在线一区| 91亚洲熟妇国产熟妇肥婆| 激情五月开心五月麻豆| 日韩精品中文字幕电影| 久草区免费在线视频播放| 国产精品97久久97久久久久| 夫妻性生活视频全过程| 成人免费毛片1000部| 亭亭丁香激情五月涩久久| 熟妇丰满多毛的大阴户| 精品女同一区二区三区久久| 欧美精品亚洲精品日韩经典| 人妻精品中文字幕一区二区在线| 国产91在线精品超碰人人| 五月天亚洲综合小说网| 97国产在线视频观看| 国产l精品国产亚洲区久久| 一区二区三区四区毛片| 亚洲精品你懂的av在线| 九九久久精品久久久精品| 欧美日韩亚洲视频一区久久| 88国产精品久久久久久| 国产又粗又长又猛又爽视频| 午夜视频免费在线观看| 99久久精品人妻少妇一| 中文字幕av久久激情| 国产日产亚洲综合一区| 亚洲精品最新地址久久久|