亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>倉(cāng)鼠肺細(xì)胞>CRL-1657R 1610 倉(cāng)鼠肺細(xì)胞

R 1610 倉(cāng)鼠肺細(xì)胞

簡(jiǎn)要描述:CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1657
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2017

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞 的詳細(xì)介紹

CRL-1657

ATCC® Number:



Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus (hamster, Chinese)

Morphology: fibroblast



Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.



Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲欧美另类国产一区| 久久香蕉国产线看观看亚洲| 欧美亚洲另类麻豆综合在线| 亚洲华人在线免费视频| 中国女人内射91熟女| 欧美一级特黄免费大片| 午夜一区二区精品视频国产| 国产黄色一区二区三区四区| 亚洲另类偷拍校园伦理| 日韩欧美一区二区免费| 伊人亚洲一区二区三区| 蜜桃人妻av一区二区三区| 青青草原在线视频观看| 少妇高潮时会抱紧男人脖子| 日韩欧美国产综合一区二区| 特黄日韩免费一区二区三区| 久久精品亚洲夜色国产av| 人妻少妇亚洲中文字幕| 国语av一区二区三区| 日韩有码大片最新自拍| 尹人大香蕉在线视频| 蜜臀久久精品国产综合| 不卡一区二区黄色av| 中国成熟女人毛茸茸视频| 在线观看国产一区二区不卡| 亚洲av乱码国产精品观看| 午夜在线观看欧美福利| 欧美日韩另类中文字幕| 日本免费一区二区三区手机在线| 人妻一区日韩二区国产| 久视频这里只有精品99| 丰满少妇一区二区三区在线观看| 国产熟女乱免费一区二区| 欧美午夜福利一级高清| 色婷婷综合中文久久一本| 伊人性伊人情亚洲综合| 国产精品女人毛片在线看| 91久久亚洲综合精品日本| 成人免费视频国产免费| 风流少妇奶真白摸的好爽| 日韩在线不卡播放视频|