亚洲欧美日韩牲夜夜综合久久-四虎永久精品在线播放-亚洲一区二区天堂在线-亚洲精品久久久久久成人爱看av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動物腎細(xì)胞>VERO 非洲綠猴腎細(xì)胞

VERO 非洲綠猴腎細(xì)胞

簡要描述:VERO 非洲綠猴腎細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2473

產(chǎn)品分類

Product Category

詳細(xì)介紹

VERO C1008 (E6)非洲綠猴腎細(xì)胞

ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

VERO 非洲綠猴腎細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1586™  Price:
Designations:VERO C1008 [Vero 76, clone E6, Vero E6]

Depositors:EM Earley

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Cercopithecus aethiops

Morphology:epithelial

Source:Organ: kidney
                       Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Virus Susceptibility:Junin virus

Machupo virus

Lassa virus

Marburg virus

Zaire Ebola virus



Comments:This is a clone of VERO 76 (ATCC CRL-1587).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                       Temperature: 37.0°C


Subculturing:Protocol:                        
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended
                       Medium Renewal: 2 to 3 times per week


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
                       Storage temperature: liquid nitrogen vapor phase


Doubling Time:22 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:26095: Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988

32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020




















 

產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日韩欧美国产精品加勒比| 最近av中文字幕电影| 蜜臀av人妻一区二区三区| 久久最新视频中文字幕| 成人黄性视频免费网看| 免费激情在线视频网址| 日韩在线不卡视频一区| 亚洲欧美另类国产一区| 欧美日韩国产这里只有精品| 懂色av中文一区二区| 欧美日本在线区一区二| 日本午夜福利久久久| 黄色av免费播放网站| 色婷婷av一区二区三区张| 欧美亚洲精品一区在线观看| 91中文字幕国产日韩| 未满十八禁止在线播放| 国产精品一级片免费看| 亚洲欧美日韩国产一区二区三区| 日本韩国三级理伦久久久| 精品一区二区三区女同| 白浆熟女精品国产91| 日韩亚洲欧洲一区二区三区| 少妇高潮毛片免费看高潮| 亚洲精品啪啪一区二区| 97在线观看视频免费| 国产H精品在线观看| 国产高清学生三级一区二区| 午夜啪视频免费在线观看| 亚洲国产欧美日韩国产| 日韩欧美精品在线不卡| 精品久久人人做爽综合| 一区二区三区av夏目彩春| av成熟一区二区三区| 欧美日本午夜福利在线观看| 日韩欧美一区二区中文字幕视频| 這裏隻有无码人妻久久| 亚洲av最近在线观看| 日韩激情av中文字幕 | 国产精品自拍国产精品| 欧美熟妇精品一级视频|